FluorFit

Developer's DescriptionBy FluorTools.comAnalyse time-resolved fluorescence decays.You can use FluorFit to: Analyse time-resolved fluorescence decays by iterative reconvolution or modified tailfitting. Perform global optimization: Analyse multiple decays simultaneously by constraining one or more variables globally. Make user defined Gaussian instrument response functions (IRFs). Fit to regular exponential decays. Fit to Gaussian lifetime-distributions. Perform time-resolved FRET analysis: Fit decays using a Gaussian distribution of donor-acceptor (D-A) distances. Fit decays using the combination of a Gaussian D-A distribution plus an imported D-A distribution, e.g. from MD simulations corresponding to local linker dynamics. Implement your own decay models (only MATLAB version). Perform parameter confidence interval estimation, either via. the Jacobian matrix or by investigating the chi-square surface. Perform an autocorrelation plot analysis. Set shift and scatter as fit parameters; subtract background counts. FluorFit accepts ASCII files as input and fits can be exported back to ASCII. Data can be loaded in units of ns or channels and in any vector direction. Plots can be modified and exported as high resolution figures. FluorFit can handle multiple figure windows in one session. FluorFit sessions can be saved and reopened. Fluorfit is particularly practical when you have multiple decays and IRFs.